Project Details
Deubiquitinating enzymes in selective autophagy (D03 (A05))
Subject Area
Biochemistry
Cell Biology
Cell Biology
Term
from 2016 to 2023
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 259130777
Dynamic protein ubiquitination plays an essential regulatory role in selective autophagy. This project aims to provide a comprehensive view on the function of deubiquitinating enzymes (DUBs) in different selective autophagy pathways. To systematically analyze DUBs that regulate mitophagy, pexophagy, and ER-phagy, we will perform a multiplex CRISPR/Cas9-based genetic screen targeting individual human DUBs and all possible combinations of DUB pairs. To study the mechanistic and functional role of identified DUBs in selective autophagy pathways, we will employ quantitative mass spectrometry-based proteomics, imaging, and biochemical methods.
DFG Programme
Collaborative Research Centres
Applicant Institution
Goethe-Universität Frankfurt am Main
Project Heads
Professorin Dr. Petra Beli; Dr. Anja Bremm