Project Details
Projekt Print View

Deubiquitinating enzymes in selective autophagy (D03 (A05))

Subject Area Biochemistry
Cell Biology
Term from 2016 to 2023
Project identifier Deutsche Forschungsgemeinschaft (DFG) - Project number 259130777
 
Dynamic protein ubiquitination plays an essential regulatory role in selective autophagy. This project aims to provide a comprehensive view on the function of deubiquitinating enzymes (DUBs) in different selective autophagy pathways. To systematically analyze DUBs that regulate mitophagy, pexophagy, and ER-phagy, we will perform a multiplex CRISPR/Cas9-based genetic screen targeting individual human DUBs and all possible combinations of DUB pairs. To study the mechanistic and functional role of identified DUBs in selective autophagy pathways, we will employ quantitative mass spectrometry-based proteomics, imaging, and biochemical methods.
DFG Programme Collaborative Research Centres
 
 

Additional Information

Textvergrößerung und Kontrastanpassung