Selective autophagy in proteostasis maintenance (D04 (A02))

• Subject Area: Biochemistry ; Cell Biology
• Term: from 2016 to 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 259130777

Building on expertise and methods established in the 1st funding period, we seek to (i) identify selective autophagy receptor-specific cargo in fed and proteostasis-challenged cells using quantitative proteomics-based autophagosome content profiling, (ii) determine the molecular grammar and machinery that drives autophagic cargo engagement under these conditions, and (iii) develop a LC3B-based reporter in mice to examine physiologically relevant autophagy cargo alterations upon proteostasis challenges in a cell type-specific manner in these animals. Overall, this project will substantially advance our understanding of proteostasis maintenance by selective autophagy pathways.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1177: Molecular and Functional Characterization of Selective Autophagy
• Applicant Institution: Goethe-Universität Frankfurt am Main
• Project Head: Professor Christian Behrends, Ph.D.