Structural characterization of domain-domain interactions in autophagy (D09 (A01))

• Subject Area: Structural Biology
• Term: from 2016 to 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 259130777

This project focuses on characterizing the interaction of non-canonical LIR sequences with members of the LC3/GABARAP autophagy modifier family and the question how specificity is achieved, for example by additional post-translational modifications. Furthermore, the interactions and structures of complexes regulating the autophagic removal of mitochondria in C. elegans will be investigated. Finally, selective binders/inhibitors for all six human LC3/GABARAP proteins will be generated and validated based on Designed Ankyrin Repeat Proteins (DARPins).

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1177: Molecular and Functional Characterization of Selective Autophagy
• Applicant Institution: Goethe-Universität Frankfurt am Main
• Project Heads: Professor Dr. Volker Dötsch; Professor Dr. Stefan Knapp, until 12/2019