Signaling mechanism, subcellular localization and regulation of guanylyl cyclases A and B, the receptors for atrial and C-type natriuretic peptides (C07)

• Subject Area: Biophysics ; Anatomy and Physiology
• Term: from 2015 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 258780946

The cardiovascular actions of atrial (ANP) and C-type natriuretic peptides (CNP) are mediated by two transmembrane cyclic GMP forming guanylyl cyclase (GC) receptors, GC-A and GC-B. To characterize the mechanism of ligand-dependent activation of cGMP production by these receptors we will use a FRET-FlAsH approach and visualize the dynamics of ligand-induced conformational changes in living cells. In addition we will apply single-molecule microscopy and superresolution imaging to compare the distinct subcellular localizations of GC-A and GC-B in healthy and hypertrophied cardiomyocytes.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 166: High-end light microscopy elucidates membrane receptor function - ReceptorLight
• Applicant Institution: Friedrich-Schiller-Universität Jena
• Co-Applicant Institution: Julius-Maximilians-Universität Würzburg
• Project Head: Professorin Dr. Michaela Kuhn