Project Details
Projekt
Characteristics of the deposition of drugs in dental biofilms
Subject Area
Toxicology, Laboratory Medicine
Pathology
Dentistry, Oral Surgery
Pathology
Dentistry, Oral Surgery
Term
since 2015
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 270621546
Large interindividual concentration differences suggest interindividual influences that affect the incorporation of drugs into biofilm. In order to utilize biofilm in forensic toxicology, it should be investigated what these concentration differences depend on. First, the optimal growing conditions for structurally different monospecies biofilms of typical oral bacteria will be determined. The extent to which the different species store different amounts of xenobiotics and at which localization within the biofilm (channels, adsorption to cell walls, or intracellular) will be determined. The dependence between duration and concentration of the exposure as well as the physicochemical properties of the xenobiotics and the stored concentrations will be investigated on species level. Furthermore, it will be investigated how long drugs are detectable in the biofilm with respect to different bacterial species after the end of a simulated drug consumption. The dependence of the concentration on the sampling site of the biofilm in the mouth, the composition of the sampled biofilm as well as intraindividual differences and time dependence will be investigated in consecutive subject studies. A special bioreactor will be used for the in vitro experiments. All biofilm samples will be examined microscopically (fluorescence microscope and/or confocal laser scanning microscope) and via instrumental analysis with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Microbial degradation products will be investigated by high-resolution mass spectrometry (LC-HR-QTOF-MS). Molecular genetic microbiome analysis will be performed using high-throughput sequencing (Next Generation Sequencing).
DFG Programme
Research Grants
