Spatial and temporal coordination of RNA translation, replication and assembly in a membranous plus-strand RNA virus replication factory (11)

• Subject Area: Virology
• Term: since 2014
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 240245660

We made important progress in identifying relevant viral and cellular factors in plus-strand RNA virus replica-tion organelle (RO) biogenesis and functionality, and revealed surprising parallels between HCV and SARS CoV 2. Furthermore, we identified a sensitive step in Dengue replication that turned out to be well suited for antiviral therapy by employing an integrated mathematical model at single-cell resolution. In the next funding period, we want to continue conducting comparative analyses of viral and host cell factors involved in RO biogenesis and function across phylogenetically distant viruses. Bioinformatics and systems biology approaches will be included to capture the dynamics of RO formation, viral replication and virion assembly.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1129: Integrative Analysis of Pathogen – Replication and Spread
• Applicant Institution: Ruprecht-Karls-Universität Heidelberg
• Project Heads: Professor Dr. Ralf Friedrich Wilhelm Bartenschlager; Professor Dr. Thomas Höfer; Professor Dr. Karl Rohr, until 6/2018