A label-free optical sensor system on silicon: Determining the thermodynamic and kinetic quantities of protein-ligand interactions
Organic Molecular Chemistry - Synthesis and Characterisation
Final Report Abstract
The main objective of this project was to develop a biochemical sensing system based on silicon photonics and the corresponding biochemical assay techniques for the detection of selected proteins. The work was divided into three subprojects: • Optical biosensor system • Surface chemistry • Assay techniques One project partner mainly worked on APTES-silanized substrates and ATR crystals, which were modified with crosslinkers and peptides. Protocols developed in this activity were used for the SOI-based sensor systems surface functionalization. The development of the label-free bio sensor system comprises of the sensor itself, a TDM- like control for fast read-out and fixed-wavelength operation. The device is based on evanescent field sensing and ring resonators as sensing elements. A heating electrode is integrated to tune the device by using the thermo-optical effect of silicon. To enable multiparameter analysis, several ring resonators were coupled to a single bus waveguide. By applying a temporal modulation signal in a fix switching scheme to each ring it was demonstrated that a serial array of ring elements could be read-out simultaneously at a speed of 100 measurements / s for each ring. This measurement speed makes the kinetics of proteinligand interaction accessible. Furthermore, the fixed wavelength operation enables the realization of a cost effective sensor device with large potential for a handheld appliance. In focus of the assay technique development we figure out a list of peptides all recognizing an anti-GST IgG antibody but with different affinities spanning from middle/high nanomolar up to micromolar affinity. Furthermore, to pave the way for a practical usage of the label-free bio sensor system we engineer a microfluidic surface functionalization and sensing technique successfully.
Publications
- Multiplexed single wavelength bio sensor for low cost applications, in Advanced Photonics 2015, OSA Technical Digest (online) (Optical Society of America, 2015), paper SeT1C.4.
M. Jäger, D. Volkmann, J. Bruns, and K. Petermann
(See online at https://doi.org/10.1364/SENSORS.2015.SeT1C.4) - Antifouling coatings on SOI microring resonators for bio sensing applications, Sensors Actuators, B Chem., vol. 223, pp. 400–405, 2016
M. Jäger, T. Becherer, J. Bruns, R. Haag, and K. Petermann
(See online at https://doi.org/10.1016/j.snb.2015.08.112) - IR-spektroskopische Charakterisierung von organisch-funktionalisierten Monolagen und Immobilisierung von Peptiden auf Silizium Oberflächen, doctoral thesis, TU Berlin, November 2018
Anne Borowski
(See online at https://doi.org/10.14279/depositonce-7790) - TDM-controlled ring resonator arrays for fast, fixed-wavelength optical biosensing, Optics Express, vol. 26, no. 17, pp. 22356–22365, 2018
P. Moock, L. Kasper, M. Jäger, D. Stolarek, H. Richter, J. Bruns, and K. Petermann
(See online at https://doi.org/10.1364/OE.26.022356)
