Project Details
Projekt
Synthetic endosomes (23)
Subject Area
Cell Biology
Term
from 2014 to 2021
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 112927078
We aim at building synthetic endosomes recapitulating the function of early endosomes. Through physically defining the organization and mechanical properties of the endosomal transport machinery, we will provide generalized insights into the structure of trafficking with a special focus on 1) the entropic collapse and its generalization beyond the endosome, 2) Rab effectors in membrane fusion, and 3) the spatial segregation on the membrane. Through a comprehensive structural and functional analysis of the critical protein and lipid species responsible for endosomal biogenesis, tethering and fusion, we generate quantitative biophysical data. We will further develop our physical model for extended tethering proteins to determine how individual proteins assemble into complex structures that physically define the endosomal transport machinery.
DFG Programme
CRC/Transregios
Applicant Institution
Ruprecht-Karls-Universität Heidelberg
Project Head
Professor Marino Zerial, Ph.D.
