Molecular dissection of anti-inflammatory effects of HDL (21)

• Subject Area: Immunology
• Term: from 2014 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 112927078

We aim to molecularly decipher how HDL exerts anti-inflammatory effects on macrophages. We will analyze PC metabolism in macrophages using two different lipidomics methods. We will inhibit phospholipases PLA1, PLA2, PLC and PLD in macrophages treated or use non-cleavable PC variants to test anti-inflammatory effects of phosphatidylcholine (PC) metabolites. Using caged PC metabolites we will then analyze the kinetics of the observed anti-inflammatory effects on a single cell level. Finally, we will profile how the PC metabolite can influence epigenetic reprogramming of macrophages and correlate this to the effects observed using reconstituted and native species.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 83: Molecular Architecture and Cellular Functions of Lipid/Protein Assemblies
• Applicant Institution: Ruprecht-Karls-Universität Heidelberg
• Project Head: Professor Dr. Eicke Latz