Project C02 has established a portfolio of quantitative mass spectrometry (MS)-based proteomics methods for studying the proteome, phosphoproteome and specific protein interaction networks of B cells. In the second funding period, we comprehensively studied the interactomes of the inhibitory receptors CD22 and Siglec-G as well as the B cell activating factor receptor, BAFFR, and the Fc fragment of IgG receptor IIb. To explore B-cell signaling networks, a new quantitative phosphoproteomics approach was implemented and then employed to elucidate the PKC signaling network in B cells. Label-free proteomics was used for quantitative studies of (primary) B cell and plasma cell proteomes. In the new funding period, multi-conditional quantitative proteomics studies in B cells will be performed to delineate dynamic changes in B-cell-specific protein interactomes, phosphoproteomes and (primary) B cell populations. C02 will enable high-end quantitative proteomics studies in numerous projects and is therefore of central importance for the TRR130.
DFG Programme
CRC/Transregios
