Chromatin regulation at promoters and enhancers, a molecular-mechanistic analysis (A02)

• Subject Area: General Genetics and Functional Genome Biology
• Term: from 2013 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 213249687

Our goal is to understand the regulation of gene expression in Drosophila development, and in particular the interplay between transcription factor activity and chromatin organization. Building on a comprehensive genome-wide analysis of core promoters, we plan to use sensitive proteomics approaches to characterize the interactome of selected general transcription machinery components and histone modifications in a promoter-type specific fashion. In a second aim, we seek to characterize the role of pioneer transcription factors in regulating chromatin accessibility at enhancers within the Ecdysone signaling cascade.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1064: Chromatin Dynamics
• Applicant Institution: Ludwig-Maximilians-Universität München
• Project Head: Professorin Dr. Ulrike Gaul (†)