EXC 81:  Cellular Networks: From Analysis of Molecular Mechanisms to a Quantitative Understanding of Complex Functions (CellNetworks)

Final Report Abstract

Within Germany, the Heidelberg Life Science community features an unparalleled number of inter-nationally renowned institutions in very close spatial proximity. Heidelberg is thus home to a critical mass of scientific and technological expertise unique to Germany, and a work force that makes it ideal for a large interdisciplinary cluster aiming at understanding the structure, function and evolu-tion of subcellular and supracellular networks. CellNetworks, founded in 2006, made use of this opportunity by assembling a large number of distinguished groups from Heidelberg molecular life science institutions, as well as specialists from mathematics, computational sciences, chemistry and physics to tackle fundamental questions surrounding network structures and architecture, dy-namics and regulation. Our approach benefitted tremendously from the combination of molecular research and computational approaches and led to a better understanding of cellular processes. A bottom-up process in defining future research topics for special funding within CellNetworks re-sulted in six thrilling projects, the results of which leveraged three new CRCs and one DFG-funded network for electron microscopy. A prerequisite of this success was the additional recruiting of excellent scientists, preferentially at disciplinary interfaces (professorship for protein evolution), or specialists in technology (professor-ships for cryo-electron microscopy and proteomics). Furthermore, ten junior research groups, funded by the cluster, and ten junior groups, funded by the Chica and Heinz Schaller Foundation supporting the cluster´s research, have created an exciting atmosphere for the next generation of scientists. Our new colleagues stimulated the technology-oriented development and dynamic inte-gration of new research questions into our research portfolio in the molecular life sciences in Hei-delberg. In parallel, the development of central core facilities for technical service, project consul-tancy and on-the-job-training became an important interlink among scientists of different disciplines and status groups within the university and our partner institutions. CellNetworks thus was, is and will be a strong driving force for the integration of research in the molecular biosciences in Heidelberg across disciplinary and institutional boundaries, to create and to continuously improve high-end core infrastructure and expertise, as well as support computa-tional approaches. We took particular care to sustain the positions of CellNetworks professorships, leading scientists at core facilities, and members of project management. We envision this invest-ment as a lasting benefit for CellNetworks members and for the life science community in Heidel-berg in general. CellNetworks will continue as central unit of the university to support core facilities in the life sciences.

Link to the final report

https://dx.doi.org/10.2314/KXP:1697276407

Publications

• Composition and three-dimensional architecture of the dengue virus replication and assembly sites. Cell Host Microbe. 2009 Apr 23;5(4):365-75
  Welsch S, Miller S, Romero-Brey I, Merz A, Bleck CK, Walther P, Fuller SD, Antony C, Krijnse- Locker J, Bartenschlager R
  
• HIV-1 Nef interferes with host cell motility by deregulation of cofilin. Cell Host Microbe. 2009 Aug 20;6(2):174-86
  Stolp B, Reichman-Fried M, Abraham L, Pan X, Giese SI, Hannemann S, Goulimari P, Raz E, Grosse R and Fackler OT
  
• Autoregulatory and repressive inputs localize Hydra Wnt3 to the head organizer. Proc Natl Acad Sci USA. 2011 May 31;108(22):9137-42
  Nakamura Y1, Tsiairis CD, Özbek S, Holstein TW
  
• Hemoglobins S and C interfere with actin remodeling in Plasmodium falciparum-infected erythrocytes. Science. 2011 Dec 2;334(6060):1283-6
  Cyrklaff M, Sanchez CP, Kilian N, Bisseye C, Simpore J, Frischknecht F, Lanzer M
  
• Nuclear calcium-VEGFD signaling controls maintenance of dendrite arborization necessary for memory formation. Neuron. 2011 Jul 14;71(1):117-30
  Mauceri D, Freitag HE, Oliveira AM, Bengtson CP, Bading H
  
• Quantitative evaluation of mechanosensing of cells on dynamically tunable hydrogels. J Am Chem Soc. 2011 Feb 9;133(5):1367-74
  Yoshikawa HY, Rossetti FF, Kaufmann S, Kaindl T, Madsen J, Engel U, Lewis AL, Armes SP and Tanaka M
  
• Maturation-dependent HIV-1 surface protein redistribution revealed by fluorescence nanoscopy. Science. 2012 Oct 26;338(6106):524-8
  Chojnacki J, Staudt T, Glass B, Bingen P, Engelhardt J, Anders M, Schneider J, Müller B, Hell SW, Kräusslich HG
  
• SAMHD1 restricts HIV-1 infection in resting CD4+ T cells. Nat Med. 2012 Nov;18(11):1682-7
  Baldauf HM, Pan X, Erikson E, Schmidt S, Daddacha W, Burggraf M, Schenkova K, Ambiel I, Wabnitz G, Gramberg T, Panitz S, Flory E, Landau NR, Sertel S, Rutsch F, Lasitschka F, Kim B, König R, Fackler OT and Keppler OT
  
• Structure and dynamics of the ATP-bound open conformation of Hsp70 chaperones. Molecular Cell. 2012 Dec 28;48(6):863-74
  Kityk R, Kopp J, Sinning I and Mayer MP
  
• Synchronizing nuclear import of ribosomal proteins with ribosome assembly. Science. 2012 Nov 2;338(6107):666-71
  Kressler D, Bange G, Ogawa Y, Stjepanovic G, Bradatsch B, Pratte D, Amlacher S, Strauß D, Yoneda Y, Katahira J, Sinning I and Hurt E
  
• Tandem fluorescent protein timers for in vivo analysis of protein dynamics. Nat. Biotechnol. 2012 Jun 24;30(7):708-14
  Khmelinskii A, Keller PJ, Bartosik A, Meurer M, Barry JD, Mardin BR, Kaufmann A, Trautmann S, Wachsmuth M, Pereira G, Huber W, Schiebel E and Knop M
  
• Detecting endogenous SUMO targets in mammalian cells and tissues. Nat Struct Mol Biol. 2013 Apr;20(4):525-31
  Becker J, Barysch SV, Karaca S, Dittner C, Hsiao HH, Berriel Diaz M, Herzig S, Urlaub S and Melchior F
  
• Robust RNAi enhancement via human Argonaute-2 overexpression from plasmids, viral vectors and cell lines. Nucleic Acids Res. 2013 Nov;41(21):e199
  Börner K, Niopek D, Cotugno G, Kaldenbach M, Pankert T, Willemsen J, Zhang X, Schürmann N, Mockenhaupt S, Serva A, Hiet MS, Wiedtke E, Castoldi M, Starkuviene V, Erfle H, Gilbert DF, Bartenschlager R, Boutros M, Binder M, Streetz K, Kräusslich HG and Grimm D
  
• AAV8-mediated in vivo overexpression of miR-155 enhances the protective capacity of genetically-attenuated malarial parasites. Mol Ther. 2014, Dec;22(12):2130-41
  Hentzschel F, Hammerschmidt-Kamper C, Börner K, Heiss K, Knapp B, Sattler JM, Kaderali L, Castoldi M, Bindman JG, Malato Y, Willenbring H, Mueller AK and Grimm D
  
• DENR•MCT-1 Promotes Translation Reinitiation Downstream of uORFs to Control Tissue Growth. Nature. 2014 Aug 14; 512(7513): 208–212
  Schleich S, Strassburger K, Janiesch PC, Koledachkina T, Miller KK, Haneke K, Cheng Y-C, Kuechler K, Stoecklin G, Duncan KE and Teleman AA
  
• FHOD1 interaction with nesprin-2G mediates TAN line formation and nuclear movement. Nat Cell Biol., 2014 Jul;16(7):708-15
  Kutscheidt S, Zhu R, Antoku S, Luxton GGW, Stagljar I, Fackler OT and Gundersen GG
  
• Genome-wide siRNA screens reveal VAMP3 as a novel host factor required for Uukuniemi virus late penetration. J Virol. 2014 Aug;88(15):8565-78
  Meier R, Franceschini A, Horvath P, Tetard M, Mancini R, von Mering C, Helenius A and Lozach PY
  
• Impaired path integration and grid cell spatial periodicity in mice lacking GluA1-containing AMPA receptors. J Neurosci. 2014 Apr 30;34(18):6245-59
  Allen K, Gil M, Resnik E, Toader O, Seeburg P, Monyer H
  
• Investigating the role of F-actin in human immunodeficiency virus assembly by live-cell microscopy. J Virol. 2014 Jul;88(14):7904-14
  Rahman SA, Koch P, Weichsel J, Godinez WJ, Schwarz U, Rohr K, Lamb DC, Kräusslich HG and Müller B
  
• Male-female communication triggers calcium signatures during fertilization in Arabidopsis. Nat Commun. 2014 Aug 22;5:4645
  Denninger P, Bleckmann A, Lausser A, Vogler F, Ott T, Ehrhardt DW, Frommer WB, Sprunck S, Dresselhaus T, Grossmann G
  
• Quantitative microscopy of functional HIV post-entry complexes reveals association of replication with the viral capsid. Elife. 2014 Dec 17;3:e04114
  Peng K, Muranyi W, Glass B, Laketa V, Yant SR, Tsai L, Cihlar T, Müller B, Kräusslich HG
  
• Sequential actions of β-catenin and Bmp pattern the oral nerve net in Nematostella vectensis. Nat Commun. 2014 Dec 23;5:5536
  Watanabe H, Kuhn A, Fushiki M, Agata K, Özbek S, Fujisawa T, Holstein TW
  
• The histone-fold protein CHRAC14 influences chromatin composition in response to DNA damage. Cell Rep. 2014 Apr 24;7(2):321-330
  Mathew V, Pauleau AL, Steffen N, Bergner A, Becker PB, Erhardt S
  
• Wnt-Fzd signaling sensitizes peripheral sensory neurons via distinct non-canonical pathways. Neuron. 2014 Jul 2;83(1):104-21
  Simonetti M, Agarwal N, Stösser S, Bali KK, Karaulanov E, Kamble R, Pospisilova B, Kurejova M, Birchmeier W, Niehrs C, Heppenstall P, Kuner R
  
• Alleviation of off-target effects from vector-encoded shRNA via co-delivered RNA decoys. Proc Natl Acad Sci USA. 2015 Jul 28;112(30):E4007-16
  Mockenhaupt S, Grosse S, Rupp D, Bartenschlager R and Grimm D
  
• Cytoadhesion of Plasmodium falciparum-infected erythrocytes to chondroitin-4-sulfate is cooperative and shear enhanced. Blood. 2015 Jan 8;125(2):383-91. doi: 10.1182/blood-2014-03-561019
  Rieger H, Yoshikawa HY, Quadt K, Nielsen MA, Sanchez CP, Salanti A, Tanaka M, Lanzer M
  
• Direct observation of ultrafast collective motions in CO myoglobin upon ligand dissociation. Science. 2015 Oct 23;350(6259):445-50
  Barends TRM, Foucar L, Ardevol A, Nass K, Aquila A, Botha S, Doak RB, Falahati K, Hartmann E, Hilpert M, Heinz M, Hoffmann MC, Köfinger J, Koglin JE, Kovacsova G, Liang M, Milathianaki D, Lemke H, Reinstein J, Roome CM, Shoeman RL, Williams GJ, Burghardt I, Hummer G, Boutet S, Schlichting I
  
• Fundamental properties of unperturbed haematopoiesis from stem cells in vivo. Nature. 2015 Feb 26;518(7540):542-6
  Busch K, Klapproth K, Barile M, Flossdorf M, Holland-Letz T, Schlenner SM, Reth M, Höfer T, Rodewald HR
  
• LncRNA Khps1 regulates expression of the proto-oncogene SPHK1 via triplex-mediated changes in chromatin structure. Mol Cell. 2015 Nov 19;60(4):626-36
  Postepska-Igielska A, Giwojna A, Gasri-Plotnitsky L, Schmitt N, Dold A, Ginsberg D, Grummt I
  
• Mammalian SRP receptor switches the Sec61 translocase from Sec62 to SRP-dependent translocation. Nat Comms. 2015 Dec 4;6:10133
  Jadhav B, McKenna M, Johnson N, High S, Sinning I and Pool MR
  
• Mechismo: predicting the mechanistic impact of mutations and modifications on molecular interactions. Nucleic Acids Research, 43.2: e10, 2015
  Betts MJ, Lu Q, Jiang YY, Drusko A, Wichmann O, Utz M, Valtierra-Gutiérrez IA, Schlesner M, Jaeger N, Jones DT, Pfister S, Lichter P, Eils R, Siebert R, Bork P, Apic G, Gavin AC, Russell RB
  
• Modulation of Presynaptic Release Probability by the Vertebrate-Specific Protein Mover. Neuron. 2015 Aug 5;87(3):521-33
  Körber C, Horstmann H, Venkatamarani V, Herrmannsdörfer F, Kremer T, Kaiser M, Schwenger DB, Ahmed S, Dean C, Dresbach T and Kuner T
  
• NS5A Domain 1 and Polyprotein Cleavage Kinetics Are Critical for Induction of Double-Membrane Vesicles Associated with Hepatitis C Virus Replication. MBio. 2015 Jul 7;6(4):e00759
  Romero-Brey I, Berger C, Kallis S, Kolovou A, Paul D, Lohmann V, Bartenschlager R
  
• The exosome is recruited to RNA substrates through specific adapter proteins. Cell. 2015 Aug 27;162(5):1029-38
  Thoms M, Thomson E, Baßler J, Gnädig M, Griesel S and Hurt E
  
• Transcriptional refractoriness is dependent on core promoter architecture. Nat Commun. 2015 Apr 8;6:6753
  Cesbron F, Oehler M, Ha N, Sancar G, Brunner M
  
• Triggering HIV polyprotein processing by light using rapid photodegradation of a tight-binding protease inhibitor. Nat Commun. 2015 Mar 9;6:6461
  Schimer J, Pavova M, Anders M, Pachl P, Sácha P, Cigler P, Weber J, Majer P, Rezácová P, Kräusslich HG, Müller B and Konvalinka J
  
• Zincfinger nuclease-based double strand breaks attenuate malaria parasites and reveal rare microhomology mediated end joining. Genome Biology. 2015 Nov 17;16:249
  Singer M, Marshall J, Heiss K, Mair GR, Grimm D, Mueller AK and Frischknecht F
  
• (2016) MYC/MIZ1-dependent gene repression inversely coordinates the circadian clock with cell cycle and proliferation. Nat Commun. 2016 Jun 24;7:11807
  Shostak A, Ruppert B, Ha N, Bruns P, Toprak UH; ICGC MMML-Seq Project, Eils R, Schlesner M, Diernfellner A, Brunner M
  
• A genetic interaction map of cell cycle regulators. Molecular Biology of the Cell, 2016
  Billmann M, Horn T, Fischer B, Sandmann T, Huber W, and Boutros M
  
• A New Population of Parvocellular Oxytocin Neurons Controlling Magnocellular Neuron Activity and Inflammatory Pain Processing. Neuron. 2016 Mar 16;89(6):1291-1304
  Eliava M, Melchior M, Knobloch-Bollmann HS, Wahis J, da Silva Gouveia M, Tang Y, Ciobanu AC, Triana Del Rio R, Roth LC, Althammer F, Chavant V, Goumon Y, Gruber T, Petit- Demoulière N, Busnelli M, Chini B, Tan LL, Mitre M, Froemke RC, Chao MV, Giese G, Sprengel R, Kuner R, Poisbeau P, Seeburg PH, Stoop R, Charlet A, Grinevich V
  
• ARHGEF17 is an essential spindle assembly checkpoint factor that targets Mps1 to kinetochores. J Cell Biol. 2016 Mar 14;212(6):647-59
  Isokane M, Walter T, Mahen R, Nijmeijer B, Hériché J-K, Miura K, Maffini S, Ivanov MP, Kitajima TS, Peters J-M and Ellenberg J
  
• Blocking sense strand activity improves potency, safety and specificity of anti-hepatitis B virus short hairpin RNA. EMBO Mol Med. 2016 Sep 1;8(9):1082-98
  Michler T, Grosse S, Mockenhaupt S, Röder N, Stückler F, Knapp B, Ko C, Heikenwälder M, Protzer U, and Grimm D.
  
• Dengue Virus Perturbs Mitochondrial Morphodynamics to Dampen Innate Immune Responses. Cell Host Microbe. 2016 Sep 14;20(3):342-356
  Chatel-Chaix L, Cortese M, Romero-Brey I, Bender S, Neufeldt CJ, Fischl W, Scaturro P, Schieber N, Schwab Y, Fischer B, Ruggieri A, Bartenschlager R
  
• Differential use of the C-type lectins L-SIGN and DC-SIGN for phlebovirus endocytosis. Traffic. 2016 Jun;17(6):639-56
  Léger P, Tetard M, Youness B, Cordes N, Rouxel RN, Flamand M, and Lozach PY
  
• Dnmt3a2: a hub for enhancing cognitive functions. Mol Psychiatry. 2016 Aug;21(8):1130-6
  Oliveira AMM, Hemstedt TJ, Freitag HE, and Bading H
  
• Expanding the Circuitry of Pluripotency by Selective Isolation of Chromatin-Associated Proteins. Mol Cell. 2016 Nov 3;64(3):624-635
  Rafiee MR, Girardot C, Sigismondo G, Krijgsveld J
  
• Force-producing ADP state of myosin bound to actin. Proc Natl Acad Sci USA. 2016 Mar 29;113(13):E1844-52
  Wulf SF, Ropars V, Fujita-Becker S, Oster M, Hofhaus G, Trabuco LG, Pylypenko O, Sweeney HL, Houdusse AM and Schröder RR
  
• Frequent mechanical stress suppresses proliferation of mesenchymal stem cells from human bone marrow without loss of multipotency. Sci Rep. 2016 Apr 15;6:24264
  Frank V, Kaufmann S, Wright R, Horn P, Yoshikawa HY, Wuchter P, Madsen J, Lewis AL, Armes SP, Ho AD and Tanaka M
  
• Integration of light and metabolic signals for stem cell activation at the shoot apical meristem. Elife. 2016 Jul 11;5. pii: e17023
  Pfeiffer A, Janocha D, Dong Y, Medzihradszky A, Schöne S, Daum G, Suzaki T, Forner J, Langenecker T, Rempel E, Schmid M, Wirtz M, Hell R, and Lohmann JU
  
• MTHFD1 controls DNA methylation in Arabidopsis. Nature Comm. 2016 Jun 13;7:11640
  Groth M, Moissiard G, Wirtz M, Wang H, Garcia-Salinas C, Ramos-Parra PA, Bischof S, Feng S, Cokus SJ, John A, Smith DC, Zhai J, Hale CJ, Long JA, Hell R, Díaz de la Garza RI, Jacobsen SE
  
• Oxidative insult can induce malaria-protective trait of sickle and fetal erythrocytes. Nat Commun. 2016 Nov 8;7:13401
  Cyrklaff M, Srismith S, Nyboer B, Burda K, Hoffmann A, Lasitschka F, Adjalley S, Bisseye C, Simpore J, Mueller AK, Sanchez CP, Frischknecht F, Lanzer M
  
• Reovirus intermediate subviral particles constitute a strategy to infect intestinal epithelial cells by exploiting TGF-β dependent pro-survival signaling. Cell. Microbiol. 2016 Dec;18(12):1831-1845
  Stanifer ML, Rippert A, Kazakov A, Willemsen J, Bucher D, Bender S, Bartenschlager R, Binder M and Boulant S
  
• Rules and Self-Organizing Properties of Post-embryonic Plant Organ Cell Division Patterns. Curr Biol. 2016 Feb 22;26(4):439-49
  von Wangenheim D, Fangerau J, Schmitz A, Smith RS, Leitte H, Stelzer EHK and Maizel A
  
• Stimulated Emission Depletion Nanoscopy Reveals Time-Course of Human Immunodeficiency Virus Proteolytic Maturation. ACS Nano. 2016 Sep 27;10(9):8215-22
  Hanne J, Göttfert F, Schimer J, Anders-Össwein M, Konvalinka J, Engelhardt J, Müller B, Hell SW, Kräusslich HG
  
• The RanBP2/Ran- GAP1SUMO1/Ubc9 SUMO E3 ligase is a disassembly machine for Crm1-dependent nuclear export complexes. Nature Comm. 2016 May 10;7:11482
  Ritterhoff T, Das H, Hofhaus G, Schröder RR, Flotho A and Melchior F
  
• Tuning a cellular lipid kinase activity adapts hepatitis C virus to replication in cell culture. Nat Microbiol. 2016 Dec19;2:16247
  Harak C, Meyrath M, Romero-Brey I, Schenk C, Gondeau C, Schult P, Esser-Nobis K, Saeed M, Neddermann P, Schnitzler P, Gotthardt D, Perez-Del-Pulgar S, Neumann-Haefelin C, Thimme R, Meuleman P, Vondran FW, De Francesco R, Rice CM, Bartenschlager R, Lohmann V
  
• Uukuniemi virus as a tick-borne virus model. J Virol. 2016 Jul 11;90(15):6784-98
  Mazelier M, Rouxel RN, Zumstein M, Mancini R, Bell-Sakyi L, and Lozach PY
  
• A unique profilin-actin interface is important for malaria parasite motility. PLoS pathog. 2017 May 26;13(5):e1006412
  Moreau CA, Bhargav SP, Kumar H, Quadt KA, Piirainen H, Strauss L, Kehrer J, Streichfuss M, Spatz JP, Wade RC, Kursula I, Frischknecht F
  
• An objective comparison of cell-tracking algorithms. Nat. Methods. 2017 Dec;14(12):1141-1152
  Ulman V, Maška M, Magnusson KEG, Ronneberger O, Haubold C, Harder N, Matula P, Matula P, Svoboda D, Radojevic M, Smal I, Rohr K, Jaldén J, Blau HM, Dzyubachyk O, Lelieveldt B, Xiao P, Li Y, Cho SY, Dufour AC, Olivo-Marin JC, Reyes-Aldasoro CC, Solis-Lemus JA, Bensch R, Brox T, Stegmaier J, Mikut R, Wolf S, Hamprecht FA, Esteves T, Quelhas P, Demirel Ö, Malmström L, Jug F, Tomancak P, Meijering E, Muñoz-Barrutia A, Kozubek M, Ortizde-Solorzano C
  
• Capturing protein communities by structural proteomics in a thermophilic eukaryote. Mol Syst Biol. 2017 Jul 25;13(7):936
  Kastritis PL, O'Reilly FJ, Bock T, Li Y, Rogon MZ, Buczak K, Romanov N, Betts MJ, Bui KH, Hagen WJ, Hennrich ML, Mackmull MT, Rappsilber J, Russell RB, Bork P, Beck M and Gavin AC
  
• Differential time-dependent volumetric and surface area changes and delayed induction of new permeation pathways in P. falciparum-infected hemoglobinopathic erythrocytes. Cellular microbiology, 2017; 19:e12650
  Waldecker M, Dasanna AK, Lansche C, Linke M, Srismith S, Cyrklaff M, Sanchez CP, Schwarz US, Lanzer M
  
• Evidence for a novel TOR sensing mechanism in higher plants. Nat Comm. 2017 Oct 27;8(1):1174
  Dong Y, Silbermann M, Speiser A, Forieri I, Linster E, Poschet G, Allboje Samami A, Wanatabe M, Sticht C, Teleman AA, Deragon JM, Saito K, Hell R, Wirtz M
  
• Genome packaging of reovirus is mediated by the scaffolding property of the microtubule network. Cell Microbiol. 2017 Dec;19(12)
  Shah PNM, Stanifer ML, Höhn K, Engel U, Haselmann U, Bartenschlager R, Kräusslich HG, Krijnse Locker J, and Boulant S
  
• Key steps in unconventional secretion of fibroblast growth factor 2 reconstituted with purified components. Elife. 2017 Jul 19;6. pii: e28985
  Steringer JP, Lange S, Čujová S, Šachl R, Poojari C, Lolicato F, Beutel O, Müller HM, Unger S, Coskun Ü, Honigmann A, Vattulainen I, Hof M, Freund C, Nickel W
  
• Loss of GET pathway orthologues in Arabidopsis thaliana causes root hair growth defects and affects SNARE abundance. Proc Natl Acad Sci USA. 2017 Feb 21;114(8):E1544-E1553
  Xing S, Mehlhorn D, Wallmeroth N, Asseck LY, Kar R, Voß A, Denninger P, Schmidt VAF, Schwarzländer M, Stierhof YD, Grossmann G and Grefen C
  
• Moltility precedes egress of malaria parasites from oocysts. Elife. 2017 Jan 24;6. pii: e19157
  Klug D and Frischknecht F
  
• Multicut brings automated neurite segmentation closer to human performance. Nat Methods. 2017 Jan 31;14(2):101-102
  Beier T, Pape C, Rahaman N, Prange T, Berg S, Bock DD, Cardona A, Knott GW, Plaza SM, Scheffer LK, Koethe U, Kreshuk A and Hamprecht FA
  
• Networks of cultured iPSC-derived neurons reveal the human synaptic activity-regulated adaptive gene program. Cell Rep. 2017 Jan 3;18(1):122-135
  Pruunsild P, Bengtson CP, Bading H
  
• Reovirus inhibits interferon production by sequestering IRF3 into viral factories. Sci Rep. 2017 Sep 7;7(1):10873
  Stanifer ML, Kischnick C, Rippert A, Albrecht D and Boulant S
  
• Rolling Adhesion of Schizont Stage Malaria-Infected Red Blood Cells in Shear Flow. Biophys J. 2017 May 9;112(9):1908-1919
  Dasanna AK, Lansche C, Lanzer M, Schwarz US
  
• SIRT7 and the DEAD-box helicase DDX21 cooperate to resolve R-loops and maintain genome stability. Genes Dev. 2017 Jul 1;31(13):1370-1381
  Song C, Hotz-Wagenblatt A, Voit R, Grummt I
  
• Sodium taurocholate cotransporting polypeptide is the limiting host factor of Hepatitis B Virus infection in macaque and pig hepatocytes. Hepatology. 2017 Sep;66(3):703-716
  Lempp FA, Wiedtke E, Qu B, Roques P, Chemin I, Vondran FW, Le Grand R, Grimm D and Urban S
  
• Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally defined protein complexes. Nat Commun. 2017 Jun 6;8:15690
  Schopp I, Amaya Ramirez C, Debeljak J, Kreibich E, Skribbe M, Wild K, and Béthune J
  
• Structural basis of HypK regulating N-terminal acetylation by the NatA complex. Nat Comms. 2017 Jun 6;8:15726
  Weyer FA, Gumiero A, Lapouge K, Bange G, Kopp J and Sinning, I
  
• Structural insights into a unique Hsp70/Hsp40 interaction in the eukaryotic ribosome-associated complex. Nat Struct Mol Biol. 2017 Feb;24(2):144-151
  Weyer FA, Gumiero A, Valentín Gesé G, Lapouge K and Sinning I
  
• Thiolutin is a zinc chelator that inhibits the Rpn11 and other JAMM metalloproteases. Nat Chem Biol. 2017 Jul;13(7):709-714
  Lauinger L, Li J, Shostak A, Cemel IA, Ha N, Zhang Y, Merkl PE, Obermeyer S, Stankovic- Valentin N, Schafmeier T, Wever WJ, Bowers AA, Carter KP, Palmer AE, Tschochner H, Melchior F, Deshaies RJ, Brunner M, Diernfellner A
  
• Ultrastructural Characterization of Zika Virus Replication Factories. Cell Rep. 2017 Feb 28;18(9):2113-2123
  Cortese M, Goellner S, Acosta EG, Neufeldt CJ, Oleksiuk O, Lampe M, Haselmann U, Funaya C, Schieber N, Ronchi P, Schorb M, Pruunsild P, Schwab Y, Chatel-Chaix L, Ruggieri A, Bartenschlager R
  
• AAV vector-mediated in vivo reprogramming into pluripotency. Nat Commun. 2018 Jul 9;9(1):2651
  Senís E, Mosteiro L, Wilkening S, Wiedtke E, Nowrouzi A, Afzal S, Fronza R, Landerer H, Abad M, Niopek D, Schmidt M, Serrano M, and Grimm D
  
• Adult hippocampal MeCP2 preserves the genomic responsiveness to learning required for long-term memory formation. Neurobiol Learn Mem. 2018 Mar;149:84-97
  Gulmez Karaca K, Brito DVC, Zeuch B, Oliveira AMM
  
• Bicoid gradient formation mechanism and dynamics revealed by protein lifetime analysis. Mol Syst Biol. 2018 Sep 4;14(9):e8355
  Durrieu L, Kirrmaier D, Schneidt T, Kats I, Raghavan S, Hufnagel L, Saunders TE and Knop M
  
• Clathrin-adaptor ratio and membrane tension regulate the flat-to-curved transition of the clathrin coat during endocytosis. Nat Commun. 2018 Mar 16;9(1):1109
  Bucher D, Frey F, Sochacki KA, Kummer S, Bergeest JP, Godinez WJ, Kräusslich HG, Rohr K, Taraska JW, Schwarz US, Boulant S
  
• Engineered anti- CRISPR proteins for optogenetic control of CRISPR-Cas9. Nat Methods. 2018 Nov;15(11):924-927
  Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Börner K, Fakhiri J, Schmelas C, Dietz L, Grimm D, Correia BE, Eils R, Niopek D
  
• Frequency Modulation of Transcription Bursting Enables Sensitive and Rapid Gene Regulation. Cell Systems. 2018 Apr 25;6(4):409- 423.e11
  Li C, Cesbron F, Oehler M, Brunner M, Höfer T
  
• Genomewide C-SWAT library for high-throughput yeast genome tagging. Nat Methods. 2018 Aug;15(8):598-600
  Meurer M, Duan Y, Sass E, Kats I, Herbst K, Buchmuller BC, Dederer V, Huber F, Kirrmaier D, Štefl M, Van Laer K, Dick TP, Lemberg MK, Khmelinskii A, Levy ED, Knop M
  
• Histone deacetylase 4 shapes neuronal morphology via a mechanism involving regulation of expression of Vascular Endothelial Growth Factor D. J Biol Chem. 2018 May 25;293(21):8196-8207
  Litke C, Bading H, Mauceri D
  
• In vivo analysis of centromeric proteins reveals a stem cell-specific asymmetry and an essential role in differentiated, non-proliferating cells. Cell Reports 2018 Feb 20;22(8):1982-1993
  Garcia Del Arco A, Edgar BA, Erhardt S
  
• Inter-subunit interactions drive divergent dynamics in mammalian and Plasmodium actin filaments. PLoS Biol. 2018 Jul 16;16(7):e2005345
  Douglas RG, Nandekar P, Aktories JE, Kumar H, Weber R, Singer M, Lepper S, Sadiq SK, Wade RC and Frischknecht F
  
• LncRNA PAPAS tethered to the rDNA enhancer recruits hypophosphorylated CHD4/NuRD to repress rRNA synthesis at elevated temperature. Genes Dev. 2018 Jun 1;32(11-12):836-848
  Zhao Z, Sentürk N, Song C, Grummt I
  
• Mechanical interactions among followers determine the emergence of leaders in migrating epithelial cell collectives. 2018 Nature Communications, 9(1):3469, 2018
  Vishwakarma M, Di Russo J, Probst D, Schwarz US, Das T and Spatz JP
  
• Molecular Mechanism of J-Domain-Triggered ATP Hydrolysis by Hsp70 Chaperones. Mol Cell. 2018 Jan 18;69(2):227-237.e4
  Kityk R, Kopp J, and Mayer MP
  
• Mutations in blind cavefish target the light-regulated circadian clock gene, period 2. Sci Rep. 2018 Jun 8;8(1):8754
  Ceinos RM, Frigato E, Pagano C, Fröhlich N, Negrini P, Cavallari N, Vallone D, Fuselli S, Bertolucci C, Foulkes NS
  
• Phenotypic memory in Bacillus subtilis links dormancy entry and exit by a spore quantityquality tradeoff. Nat Commun. 2018 Jan 4;9(1):69
  Mutlu A, Trauth S, Ziesack M, Nagler K, Bergeest JP, Rohr K, Becker N, Höfer T, Bischofs IB
  
• Rapid cue-specific remodeling of the nascent axonal proteome. Neuron. 2018 Jul 11;99(1):29-46.e4
  Cagnetta R, Frese CK, Shigeoka T, Krijgsveld J, Holt CE
  
• Secretion of Hepatitis C Virus Replication Intermediates Reduces Activation of Toll-Like Receptor 3 in Hepatocytes. Gastroenterology. 2018 Jun;154(8):2237- 2251.e16
  Grünvogel O, Colasanti O, Lee JY, Klöss V, Belouzard S, Reustle A, Esser-Nobis K, Hesebeck-Brinckmann J, Mutz P, Hoffmann K, Mehrabi A, Koschny R, Vondran FWR, Gotthardt D, Schnitzler P, Neumann-Haefelin C, Thimme R, Binder M, Bartenschlager R, Dubuisson J, Dalpke AH, Lohmann V
  
• SHRED is a regulatory cascade that reprograms Ubr1 substrate specificity for enhanced protein quality control during stress. Mol Cell. 2018 Jun 21;70(6):1025-1037.e5
  Szoradi T, Schaeff K, Garcia-Rivera EM, Itzhak DN, Schmidt RM, Bircham PW, Leiss K, Diaz- Miyar J, Chen VK, Muzzey D, Borner GH and Schuck S
  
• CH, Nagler K, Tsaprailis G, Anders S, Bischofs I, Maupin-Furlow JA, Spahn CMT, Joazeiro CAP. Alanine Tails Signal Proteolysis in Bacterial Ribosome-Associated Quality Control. Cell. 2019 Jun 27;178(1):76- 90.e22
  Lytvynenko I, Paternoga H, Thrun A, Balke A, Müller TA, Chiang CH, Nagler K, Tsaprailis G, Anders S, Bischofs I, Maupin-Furlow JA, Spahn CMT, Joazeiro CAP
  
• Destinct RopGEFs successively drive polarization and outgrowth of root hairs. Curr Biol. 2019 Jun 3;29(11):1854-1865.e5
  Denninger P, Reichelt A, Schmidt VAF, Mehlhorn DG, Asseck LY, Stanley CE, Keinath NF, Evers JF, Grefen C, Grossmann G
  
• Glutamatergic synaptic input to glioma cells drives brain tumour progression. Nature. 2019 Sep 18;573(7775):532-538
  Venkataramani V, Tanev DI, Strahle C, Studier-Fischer A, Fankhauser L, Kessler T, Körber C, Kardorff M, Ratliff M, Xie R, Horstmann H, Messer M, Paik SP, Knabbe J, Sahm F, Kurz FT, Acikgöz AA, Herrmannsdörfer F, Agarwal A, Bergles DE, Chalmers A, Miletic H, Turcan S, Mawrin C, Hänggi D, Liu HK, Wick W, Winkler F, Kuner T
  
• Illuminating G-protein coupling selectivity of GPCRs. Cell. 2019 Jun 13;177(7):1933-1947.e25
  Inoue A, Raimondi F, Kadji FMN, Singh G, Kishu T, Uwamizu A, Ono Y, Shinjo Y, Ishida S, Arang N, Kawakami K, Gutkind JS, Aoki J, Russell R
  
• Therbalance between KIFC3 and EG5 tetrameric kinesins controls the onset of mitotic spindle assembly. Nat. Cell Biol. 2019 Sep;21(9):1138-1151
  Hata S, Pastor Peidro A, Panic M, Liu P, Atorino E, Funaya C, Jäkle U, Pereira G, and Schiebel E