The main goal of the proposed studies was to characterize the role of the RO60 protein (TROVE2) in cancer-derived cells. These studies settled on the finding that the transient depletion of RO60 by siRNA pools impaired the p53-dependent upregulation of p21 synthesis. Although these findings could not be confirmed probably due to off-target effects, the studies led to the characterization of a novel role of the RO60-associated Y RNAs. For the Y3** ncRNA derived from the RO60-associated Y3 RNA we could identify a role in the 3'-end processing of canonical histone mRNAs. Moreover, the analyses set the stage for addressing the role of RO60-associated cytoplasmic Y RNAs in modulating the role of RNA-binding proteins controlling mRNA translation and/or turnover currently funded by the SPP1935. Collaborative efforts with the Quattrone lab (Trento, Italy) support the initial hypothesis proposing a role of Y RNAs in serving as decoys of specific RNA-binding proteins.