TIRF Mikroskop mit FRAP und Hochauflösung

• Subject Area: Basic Research in Biology and Medicine
• Term: Funded in 2013
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 234324669

• Final Report Year: 2017

Final Report Abstract

3D SIM imaging of the foci resulting from the DNA double-strand break repair complex Ku80 was used to show p97 mediates the release of trapped Ku80 rings. In a study of the role that CEACAM1-3S plays in driving melanoma cells into NK mediated cytolysis, SIM imaging was used to resolve the localization of CEACAM isoforms in vesicle-like structures at the sites of cell-cell contact.

Publications

• „CEACAM1-3S drives melanoma cells into NK cellmediated cytolysis and enhances patient survival.“ Cancer Res. 2015
  Ullrich N, Heinemann A, Nilewski E, Scheffrahn I, Klode J, Scherag A, Schadendorf D, Singer BB, Helfrich I
  (See online at https://doi.org/10.1158/0008-5472.CAN-14-1752)
• „Sex-Dependent Claudin-1 Expression in the Liver of Euthyroid and Hypothyroid Mice.” Eur Thyroid J. 2015
  Zwanziger D, Rakov H, Engels K, Moeller LC, Führer D
  (See online at https://doi.org/10.1159/000431316)
• „Virus-Induced Type I Interferon Deteriorates Control of Systemic Pseudomonas Aeruginosa Infection.“ Cell Physiol Biochem. 2015
  Merches K, Khairnar V, Knuschke T, Shaabani N, Honke N, Duhan V, Recher M, Navarini AA, Hardt C, Häussinger D, Tümmler B, Gulbins E, Futerman AH, Hoffmann D, Lang F, Lang PA, Westendorf AM, Lang KS
  (See online at https://doi.org/10.1159/000430200)
• “Age-Dependent Changes of Monocarboxylate Transporter 8 Availability in the Postnatal Murine Retina.“ Front. Cell. Neurosci., 2016
  Yoshiyuki Henning and Karol Szafranski
  (See online at https://doi.org/10.3389/fncel.2016.00205)
• „Neuroendocrine cells of the prostate derive from the neural crest.“ J Biol Chem. 2016
  Szczyrba J, Niesen A, Wagner M, Wandernoth PM, Aumüller G, Wennemuth G
  (See online at https://doi.org/10.1074/jbc.M116.755082)
• „VCP/p97 Extracts Sterically Trapped Ku70/80 Rings from DNA in Double-Strand Break Repair.“ Mol Cell. 2016 64(1)
  van den Boom J, Wolf M, Weimann L, Schulze N, Li F, Kaschani F, Riemer A, Zierhut C, Kaiser M, Iliakis G, Funabiki H, Meyer H
  (See online at https://doi.org/10.1016/j.molcel.2016.08.037)
• „Biofilm formation of the black yeast-like fungus Exophiala dermatitidis and its susceptibility to antiinfective agents.“ Sci Rep. 2017
  Kirchhoff L, Olsowski M, Zilmans K, Dittmer S, Haase G, Sedlacek L, Steinmann E, Buer J, Rath PM, Steinmann J
  (See online at https://doi.org/10.1038/srep42886)