Spektral-Konfokalmikroskop mit Weißlichtlaser, FLIM und Hochauflösung

• Subject Area: Basic Research in Biology and Medicine
• Term: Funded in 2013
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 234323630

• Final Report Year: 2017

Final Report Abstract

STED was applied to quantify the colocalization and number of post synaptic complexes in cultured neurons, where it was shown the number of complexes is greater in completed synapses. An imagej plugin was developed for the quantification.

Publications

• Colocalization of synapse marker proteins evaluated by STED- microscopy reveals patterns of neuronal synapse distribution in vitro. J Neurosci Methods. 2016
  Dzyubenko E, Rozenberg A, Hermann DM, Faissner A
  (See online at https://doi.org/10.1016/j.jneumeth.2016.09.001)
• “Efficient nucleic acid delivery to murine regulatory T cells by gold nanoparticle conjugates.“. Sci Rep. 2016
  Gamrad L, Rehbock C, Westendorf AM, Buer J, Barcikowski S, Hansen W
  (See online at https://doi.org/10.1038/srep28709)