We will comparatively study the protonation dynamics of two different channelrhodopsins, CaChR1 and GtACR1, the inverted proton pump NsXeR and the chloride pump NmHR in a combination of mutagenesis, time-resolved UV/Vis-, steady-state FTIR difference spectroscopy and structural methods. Local electrical field changes will be observed via VSE probes inserted into the two pumps and into phytochromes. In the complex of HsSRI and HsHtrI, we will study the structural changes involved in signal transfer.
DFG Programme
Collaborative Research Centres