GRK 1947:  Biochemical, Biophysical and Biomedical Effects of Reactive Oxygen and Nitrogen Species on Biological Membranes

• Subject Area: Basic Research in Biology and Medicine
• Term: from 2014 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 231396381

• Final Report Year: 2021

Final Report Abstract

Since the last progress report from March 2018, in total eleven doctoral researchers were employed. Another one was funded by the University to strengthen the research programme of the RTG, this doctoral researcher was supported by the RTG 1947 with consumables like all other member of the RTG. Additionally, 13 doctoral researchers funded through other sources became associated members to enrich the scientific content and in turn benefit from both the scientific and qualification programme of the RTG 1947. The scientific program consisted of lab courses, both scientific and soft-skill workshops as well as annual meetings in 2018 and 2019. In the meantime, almost all of the funded doctoral students of the first cohort have graduated, and seven of the second cohort have submitted their dissertations. Furthermore, eight of the associated doctoral researchers have finished their dissertation. Up to now, the doctoral researchers have authored 80 peer-reviewed publications. The focus of the four projects in section A was on the endogenous production of ROS and RNS that subsequently act on biological membranes and other compounds. The sources of the ROS/RNS investigated were enzymes (A2-4) and xenobiotics (A1). Significant progress was made, for instance, with respect to the redox regulation of proteins through the specific oxidation of proteins by dedicated enzymes, the structural analysis of a plastic-degrading enzyme, the regulation of the systemic Inflammatory response by the NLRP3 inflammasome in mice with acute pancreatitis, and the role of an aquaporin as crucial hydrogen peroxide transporter in insulin-producing cells. The five projects of section B focussed on the physico-chemical and chemical level of interactions of ROS/RNS with membranes. Information on changes of the physical properties of model membranes was provided such as membrane thickness, phase states, homogeneity (B1, B6) and cell elasticity (B5) as influenced by chemical reactions (B2, B4, B6) of the different membrane molecules. The protective effect of sphingomyelin on other membrane lipids was demonstrated. Methods to measure the cell elasticity were optimized. In collaboration with project C2, B2 and A3, the effect of enzyme Tafazzin on cell elasticity was explored. With electrochemistry, the thermodynamics of membrane constituents were elucidated. The three projects of project area C are focussed on the physiological and pathophysiological consequences that result from distinct interactions of ROS/RNS with membranes and essential membrane constituents in biological systems. This biological context comprised bacteria (C4, Chlostridium difficile), mammalian cells and tissues (C2), and plants (C3). Some of the most notable progress was made with respect to Tafazzindependent cardiolipin composition in mitochondrial membranes, the regulation of a plant H+-ATPase by redox modification of a specific cysteine residue, and the role of the transcriptional repressor PerR in oxidative stress response in Clostridioides difficile.

Publications

• Interactions of mono-and divalent cations with cardiolipin monolayers
  Renko Kensbock
  
• Adaptive immune response during acute pancreatitis is regulated by NLRP3 inflammasome activation via interleukin 18. Pancreatology 2018, 18(4), S87-S88
  Sendler, M.; van den Brandt, C.; Glaubitz, J.; Wilden, A.; Weiss, F. U.; Golchert, J.; Homuth, G.; Bossaller, L.; Mayerle, J.; Lerch, M. M.
  (See online at https://doi.org/10.1016/j.pan.2018.05.236)
• Biochemical characterization of an ulvan lyase from the marine flavobacterium Formosa agariphila KMM 3901 (T). Applied Microbiology and Biotechnology 2018, 102 (16), 6987-6996
  Reisky, L.; Stanetty, C.; Mihovilovic, M. D.; Schweden T.; Hehemann, J.-H.; Bornscheuer, U. T.
  (See online at https://doi.org/10.1007/s00253-018-9142-y)
• Cathepsin B-Mediated Activation of Trypsinogen in Endocytosing Macrophages Increases Severity of Pancreatitis in Mice. Gastroenterology 2018, 154 (3), 704-718
  Sendler, M.; Weiss, F. U.; Golchert, J.; Homuth, G.; van den Brandt, C.; Mahajan, U. M.; Partecke, L.-I.; Döring, P.; Gukovsky, I.; Gukovskaya, A. S.; Wagh, P. R.; Lerch, M. M.; Mayerle, J.
  (See online at https://doi.org/10.1053/j.gastro.2017.10.018)
• Depletion of CD4+ T-helper cells reduces pancreatic damage in two models of acute pancreatitis. Pancreatology 2018, 18 (4), S30
  Glaubitz, J.; Wilden, A.; van den Brandt, C.; Weiss, F. U.; Mayerle, J.; Lerch, M. M.; Sendler, M.
  (See online at https://doi.org/10.1016/j.pan.2018.05.080)
• Effects of siRNA-dependent knock-down of cardiolipin synthase and tafazzin on mitochondria and proliferation of glioma cells. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids 2018, 1863 (4), 379-387
  Ohlig, T.; Le, D. V.; Gardemann, A.; Wolke, C.; Gürtler, S.; Peter, D.; Schild, L; Lendeckel, U.
  (See online at https://doi.org/10.1016/j.bbalip.2018.01.003)
• Immune Cell and Stromal Signature Associated With Progression-Free Survival of Patients With Resected Pancreatic Ductal Adenocarcinoma. Gastroenterology 2018, 155(5), 1625-1639
  Mahajan, U. M.; Langhoff, E.; Goni, E.; Costello, E.; Greenhalf, W.; Halloran, C.; Omnanns, S.; Kruger, S.; Boeck, S.; Ribback, S.; Beyer, G.; Dombroswki, F.; Weiss, F. U.; Neoptolemos, J. P.; Werner, J.; D'Haese, J. G.; Bazhin, A.; Peterhansl, J.; Pichlmeier, S.; Büchler, M. W.; Kleeff, J.; Ganeh, P.; Sendler, M.; Palmer, D. H.; Kohlmann, T.; Rad, R.; Regel, I.; Lerch, M. M.; Mayerle, J.
  (See online at https://doi.org/10.1053/j.gastro.2018.08.009)
• Impact of gold-1- decanethiol-SAM formation and removal cycles on the surface properties of polycrystalline gold and SAM quality. Journal of Solid State Electrochemistry 2018, 22 (4), 1149-1154
  Thai, D.; Kahlert, H.; Chinnaya, J.; Ahrens, P.; Hasse, U.
  (See online at https://doi.org/10.1007/s10008-017-3858-y)
• Inhibition of calpain-1 stabilizes TCF11/Nrf1 but does not affect its activation in response to proteasome inhibition. Bioscience Reports 2018, 38
  Nowak, K.; Taubert, R. M.; Haberecht, S.; Venz, S ; Krueger, E.
  (See online at https://doi.org/10.1042/bsr20180393)
• Investigation of the antioxidative and radical scavenging impact of natural compounds in-vitro and on cell metabolism
  Dana Thal
  
• Investigation on the general activity and uncoupling of the catalytic cycle of different cytochrome P450 monooxygenase
  Lisa Morlock
  
• Light-induced intracellular hydrogen peroxide generation through genetically encoded photosensitizer KillerRed- SOD1. Free Radical Research 2018, 52 (10), 1170-1181
  Laporte, A.; Nordenbrock, A.; Lenzen, S.; Eisner, M.
  (See online at https://doi.org/10.1080/10715762.2018.1540042)
• Metabolic biomarker signature to differentiate pancreatic ductal adenocarcinoma from chronic pancreatitis. Gut 2018, 67(1), 126-137
  Mayerle, J.; Kalthoff, H.; Reszka, R.; Kamlage, B.; Peter, E.; Schniewind, B.; Maldonado, S. G.; Pilarsky, C.; Heidecke, C. D.; Schatz, P.; Distier, M.; Scheiber, J. A.; Mahajan, U. M.; Weiss, F. U.; Grutzmann, R.; Lerch, M. M.
  (See online at https://doi.org/10.1136/gutjnl-2016-312432)
• Metabolic inventory of Streptococcus pneumoniae growing in a chemical defined environment. International Journal of Medical Microbiology 2018, 308 (6), 705-712
  Leonard, A.; Gierok, P.; Methling, K.; Gómez-Mejia, A.; Hammerschmidt, S.; Lalk, M.
  (See online at https://doi.org/10.1016/j.ijmm.2018.01.001)
• Non classical macrophage polarization regulates the immune response during acute pancreatitis. Pancreatology 2018, 18(4), S126-S127
  Wilden, A.; Glaubitz, J.; Golchert, J.; Homuth, G.; Weiss, F. U.; Mayerle, J.; Lerch, M. M.; Sendler, M.
  (See online at https://doi.org/10.1016/j.pan.2018.05.340)
• Nucleoredoxin-Dependent Targets and Processes in Neuronal Cells. Oxidative Medicine and Cellular Longevity 2018
  Urbainsky, C.; Nolker, R.; Imber, M.; Lübken, A.; Mostertz, J.; Hochgräfe, F.; Godoy, J. R.; Hanschmann, E. M.; Lillig, C. H.
  (See online at https://doi.org/10.1155/2018/4829872)
• Oxidative demethylation of algal carbohydrates by cytochrome P450 monooxygenases. Nature Chemical Biology 2018, 14 (9), 342-344
  Reisky, L.; Buchsenschutz, H. C.; Engel, J.; Song, T.; Schweden T.; Hehemann, J.-H.; Bornscheuer, U. T.
  (See online at https://doi.org/10.1038/s41589-018-0005-8)
• Pinkl-Parkin mediated mitophagy in acute pancreatitis. Pancreatology 2018, 18(4), S29
  Wagh, P. R.; Sendler, M.; Mahajan, U. M.; Jhon, D.; Weiss, F. U.; Lerch, M. M.; Mayerle, J.
  (See online at https://doi.org/10.1016/j.pan.2018.05.078)
• Prolactin protects beta cells against oxidative stress through HSPB1. Diabetologia 2018, 61, S207-S207
  Labriola, L.; Wailemann, R. A. M.; Dos Santos, A. F.; Gómes, V. M.; Silva, R. P.; Laporte, A.; Meotti, F. C.; Terra, W. R.; Palmisano, G.; Lortz, S.; Terra, L. F.
  (See online at https://doi.org/10.1007/s00125-018-4687-y)
• Protein Engineering of the Progesterone Hydroxylating P450-Monooxygenase CYP17A1 Alters Its Regioselectivity. ChemBioChem 2018, 19(18), 1954-1958
  Morlock, L. K.; Grobe, S.; Balke, K.; Mauersberger, S.; Böttcher, D.; Bornscheuer, U. T.
  (See online at https://doi.org/10.1002/cbic.201800371)
• Protein S-Bacillithiolation Functions in Thiol Protection and Redox Regulation of the Glyceraldehyde-3-Phosphate Dehydrogenase Gap in Staphylococcus aureus Under Hypochlorite Stress. Antioxidants & Redox Signaling 2018, 28 (6), 410-430
  Imber, M.; Nguyen Thi Thu, H.; Pietrzyk-Brzezinska, A. J.; Vu Van, L.; Hillion, M.; Bernhardt, J.; Thaerichen, L.; Kolsek, K.; Saleh, M.; Hamilton, C. J.; Adrian, L; Graeter, F.; Wahl, M. C.; Antelmann, H.
  (See online at https://doi.org/10.1089/ars.2016.6897)
• Simultaneous detection of NADPH consumption and H202 production using the Ampliflu (TM) Red assay for screening of P450 activities and uncoupling. Applied Microbiology and Biotechnology 2018, 102 (2), 985-994
  Morlock, L. K.; Boettcher, D.; Bornscheuer, U. T.
  (See online at https://doi.org/10.1007/s00253-017-8636-3)
• The aldehyde dehydrogenase AldA contributes to the hypochlorite defense and is redox-controlled by protein S-bacillithiolation in Staphylococcus aureus. Redox Biology 2018, 15, 557-568
  Imber, M.; Vu Van, L.; Reznikov, S.; Fritsch, V. N.; Pietrzyk-Brzezinska, A. J.; Prehn, J.; Hamilton, C.; Wahl, M. C.; Bronowska, A. K.; Antelmann, H.
  (See online at https://doi.org/10.1016/j.redox.2018.02.001)
• The electrochemistry of DPPH in three-phase electrode systems for ion transfer and ion association studies. Journal of Electroanalytical Chemistry 2018, 823, 765-772
  Dharmaraj, K.; Nasri, Z.; Kahlert, H.; Scholz, F.
  (See online at https://doi.org/10.1016/j.jelechem.2018.06.012)
• Thiol-redox proteomics of Mycobacterium smegmatis in response to ROS, RNS and antibiotics
  Marcel Imber
  
• Thiol-Redox Proteomics to Study Reversible Protein Thiol Oxidations in Bacteria. In Microbial Proteomics: Methods and Protocols, Becher, D., Ed. 2018; Vol. 1841, pp 261-275
  Rossius, M.; Hochgräfe, F.; Antelmann, H.
  (See online at https://doi.org/10.1007/978-1-4939-8695-8_18)
• Tracking gene expression and oxidative damage of O-2-stressed Clostridioides difficile by a multi-omics approach. Anaerobe 2018, 53, 94-107
  Neumann-Schaal, M.; Metzendorf, N. G.; Troitzsch, D.; Nuss, A. M.; Hofmann, J. D.; Beckstette, M.; Dersch, P.; Otto, A.; Sievers, S.
  (See online at https://doi.org/10.1016/j.anaerobe.2018.05.018)
• Differential View on the Bile Acid Stress Response of Clostridioides difficile. Frontiers in Microbiology 2019, 10, 258
  Sievers, S.; Metzendorf, N. G.; Dittmann, S.; Troitzsch, D.; Gast, V.; Tröger, S. M.; Wolff, C.; Zühlke, D.; Hirschfeld, C.; Schlüter, R.; Riedel, K.
  (See online at https://doi.org/10.3389/fmicb.2019.00258)
• Drug-induced activation of integrin alpha lib beta 3 leads to minor localized structural changes. Plos One 2019, 14 (4), e0214969
  Janke, U.; Kulke, M.; Buchholz, I.; Geist, N.; Langel, W.; Delcea, M.
  (See online at https://doi.org/10.1371/journal.pone.0214969)
• Functional characterization of human class I and class II mitochondrial glutaredoxins
  Daniel Trnka
  
• Heat shock protein B1 is a key mediator of prolactin-induced beta-cell cytoprotection against oxidative stress. Free Radical Biology and Medicine 2019, 134, 394-405
  Terra, L. F.; Wailemann, R. A. M.; dos Santos, A. F.; Gomes, V. M.; Silva, R. P.; Laporte, A.; Meotti, F. C.; Terra, W. R.; Palmisano, G.; Lortz, S.; Labriola, L.
  (See online at https://doi.org/10.1016/j.freeradbiomed.2019.01.023)
• Influence of argon ion beam etching and thermal treatment on polycrystalline and single crystal gold electrodes Au(100) and Au(111). Journal of Electroanalytical Chemistry 2019, 832, 233-240
  Ahrens, P.; Zander, M.; Hirsch, D.; Hasse, U.; Wulff, H.; Frost, F.; Scholz, F.
  (See online at https://doi.org/10.1016/j.jelechem.2018.10.066)
• Interactions of monovalent and divalent cations with cardiolipin monolayers. Langmuir 2019, 35(10), 3624-3633
  Kensbock, R.; Ahrens, H.; Helm, C. A.
  (See online at https://doi.org/10.1021/acs.langmuir.8b03637)
• Investigation of the pH and temperature dependent properties of tetramyristoyl cardiolipin and its behavior in model membranes
  Marina Sturm
  
• Parkin and PINK1 mitigates severity of acute pancreatitis and accelerates recovery of exocrine pancreas
  Preshit R. Wagh
  
• Pro-inflammation and Anti-inflammation During Severe Acute Pancreatitis Develop in Parallel and Result in Misbalanced T-cell Activation Which Increases Disease Severity. Pancreas 2019, 48(10), 1518-1518
  Sendler, M.; Wilden, A.; Glaubitz, J.; van der Brandt, C.; Weiss, F. U.; Mayerle, J.; Lerch, M. M.
  
• Redox regulation by reversible protein S-thiolation in Gram-positive bacteria. Redox Biology 2019, 20, 130-145
  Imber, M.; Pietrzyk-Brzezinska, A. J.; Antelmann, H.
  (See online at https://doi.org/10.1016/j.redox.2018.08.017)
• Role of endoplasmic reticulum stress and protein misfolding in disorders of the liver and pancreas. Advances in Medical Sciences 2019, 64 (2), 315-323
  Lukas, J.; Pospech, J.; Oppermann, C.; Hund, C.; Iwanov, K.; Pantoom, S.; Petters, J.; Frech, M.; Seemann, S.; Thiel, F.-G.; Modenbach, J.-M.; Boismann, R.; Chama, L. d. F.; Kraatz, F.; El-Hage, F.; Gronbach, M.; Klein, A.; Mueller, R.; Salloch, S.; Weiss, F.-U.; Simon, P.; Wagh, P.; Klemenz, A.; Krüger, E.; Mayerle, J.; Delcea, M.; Kragl, U.; Beller, M.; Rolfs, A.; Lerch, M. M.; Serieller, M.
  (See online at https://doi.org/10.1016/j.advms.2019.03.004)
• Site-specific intracellular generation and detection of hydrogen peroxide and protection against oxidative stress in insulin-producing cells
  Anna Laporte
  
• Structure of the plasticdegrading Ideonella sakaiensis MHETase bound to a substrate. Nature Communications 2019, 10 (1), 1-10
  Palm, G. J.; Reisky, L; Böttcher, D.; Müller, H.; Michels, E. A. P.; Walczak, M. C.; Bemdt, L; Weiss, M. S.; Bornscheuer, U. T.; Weber, G.
  (See online at https://doi.org/10.1038/s41467-019-09326-3)
• Structure-toxicity relationships of saturated and unsaturated free fatty acids for elucidating the lipotoxic effects in human EndoC-beta H1 beta-cells. Biochimica et Biophysica Acta - Molecular Basis of Disease 2019, 1865 (11), 165525
  Plötz, T.; von Hanstein, A. S.; Krümmel, B.; Laporte, A.; Mehmeti, I.; Lenzen, S.
  (See online at https://doi.org/10.1016/j.bbadis.2019.08.001)
• Substrate specificity of thioredoxins and glutaredoxins - towards a functional classification. Heliyon 2019, 5 (12), e02943
  Gellert, M.; Hossaln, M. F.; Berens, F. J. F.; Bruhn, L. W.; Urbainsky, C.; Liebscher, V.; Lillig, C. H.
  (See online at https://doi.org/10.1016/j.heliyon.2019.e02943)
• Tafazzin-dependent cardiolipin composition in C6 glioma cells correlates with changes in mitochondrial and cellular functions, and cellular proliferation. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids 2019, 1864 (4), 452-465
  Gürtler, S.; Wolke, C.; Otto, O.; Heise, N.; Scholz, F.; Laporte, A.; Eisner, M.; Joems, A.; Weinert, S.; Döring, M.; Jansing, S.; Gardemann, A.; Lendeckel, U.; Schild, L.
  (See online at https://doi.org/10.1016/j.bbalip.2019.01.006)
• The Influence of Calcium Traces in Ultrapure Water on the Lateral Organization in Tetramyristoyl Cardiolipin Monolayers. ChemPhysChem 2019, 20(11), 1521-1526
  Sturm, M.; Gutowski, O.; Brezesinski, G.
  (See online at https://doi.org/10.1002/cphc.201900126)
• Vitamin B6 deficiency in new bom rats affects hepatic cardiolipin composition and oxidative phosphorylation. Experimental Biology and Medicine 2019, 244 (18), 1619-1628
  Wolke, C.; Gürtler, S.; Peter, D.; Weingärtner, J.; Domanska, G.; Lendeckel, U.; Schild, L.
  (See online at https://doi.org/10.1177/1535370219889880)
• A combination of electrochemistry and mass spectrometry to monitor the interaction of reactive species with supported lipid bilayers. Scientific Reports 2020, 10 {1), 1-15; Erratum Scientific Reports 2021, 11 (1), 1
  Ravandeh, M.; Kahlert, H.; Jablonowski, H.; Lackmann, J. W.; Striesow, J.; Hernandez, V. A.; Wende, K.
  (See online at https://doi.org/10.1038/s41598-020-75514-7 https://doi.org/10.1038/s41598-021-91696-0)
• Biopolymer-coated gold nanoparticles inhibit human insulin amyloid fibrillation. Scientific Reports 2020, 10(1), 1-14
  Meesaragandla, B.; Karanth, S.; Janke, U.; Delcea, M.
  (See online at https://doi.org/10.1038/s41598-020-64010-7)
• Changing surface properties of artificial lipid membranes at the interface with biopolymer coated gold nanoparticles under normal and redox conditions. Biophysical Chemistry 2020, 267, 106465
  Karanth, S.; Meesaragandla, B.; Delcea, M.
  (See online at https://doi.org/10.1016/j.bpc.2020.106465)
• Experimental pancreatitis is characterized by rapid T cell activation, Th2 differentiation that parallels disease severity, and improvement after CD4(+) T cell depletion. Pancreatology 2020, 20 (8), 1637-1647
  Glaubitz, J.; Wilden, A.; van den Brandt, C.; Weiss, F. U.; Bröker, B. M.; Mayerie, J.; Lerch, M. M.; Sendler, M.
  (See online at https://doi.org/10.1016/j.pan.2020.10.044)
• High-throughput cell and spheroid mechanics in virtual fluidic channels. Nature Communications 2020, 11, 1-11
  Panhwar, M. H.; Czerwinski, F.; Dabbiru, V. A. S.; Komaragiri, Y.; Fregin, B.; Biedenweg, D.; Nestler, P.; Pires, R. H.; Otto, O.
  (See online at https://doi.org/10.1038/s41467-020-15813-9)
• Highly selective bile acid hydroxylation by the multifunctional bacterial P450 monooxygenase CYP107D1 (OleP). Biotechnology Letters 2020, 42 (5), 819-824
  Grobe, S.; Wszolek, A.; Brundiek, H.; Fekete, M.; Bornscheuer, U. T.
  (See online at https://doi.org/10.1007/s10529-020-02813-4)
• Hydrogen peroxide permeability of cellular membranes in insulin-producing cells. Biochimica et Biophysica Acta - Biomembranes 2020, 1862 (2), 183096
  Laporte, A; Lortz, S.; Schaal, C.; Lenzen, S.; Eisner, M.
  (See online at https://doi.org/10.1016/j.bbamem.2019.183096)
• Interaction of fibrinogen-magnetic nanoparticle bioconjugates with integrin reconstituted into artificial membranes. Nanoscale 2020, 12(38), 19918-19930
  Martens, U.; Janke, U.; Möller, S.; Talbot, D.; Abou-Hassan, A.; Delcea, M.
  (See online at https://doi.org/10.1039/d0nr04181e)
• Intestinal Microbiota Composition is Regulated by a Suppressive Immune Response During Severe Acute Pancreatitis. Pancreas 2020, 49 (10), 1428-1428
  Sendler, M.; Wilden, A.; Glaubitz, J.; Frost, F.; Weiss, F. U.; Lerch, M. M.
  
• Label-Free Cytometry in Virtual Fluidic Channels - High-Throughput Cell Rheology and Tissue Mechanics. Biophysical Journal 2020, 118 (3), 603A-603A
  Panhwar, M. H.; Czerwinski, F.; Fregin, B.; Dabbiru, V. A.; Komaragiri, Y.; Biedenweg, D.; Pires, R. H.; Otto, O.
  (See online at https://doi.org/10.1016/j.bpj.2019.11.3257)
• Molecular basis for the distinct functions of redox-active and FeS-transfering glutaredoxins. Nature Communications 2020, 11 (1), 1-12
  Trnka, D.; Engelke, A. D.; Geliert, M.; Moseler, A.; Hossain, M. F.; Lindenberg, T. T.; Pedroletti, L.; Odermatt, B.; de Souza, J. V.; Bronowska, A. K.; Dick, T. P.; Mühlenhoff, U.; Meyer, A. J.; Bemdt, C.; Lillig, C. H.
  (See online at https://doi.org/10.1038/s41467-020-17323-0)
• Nitrosative stress affects the interaction of integrin alphallbbeta3 with its ligands. Biochimica et Biophysica Acta - Biomembranes 2020, 1862 (5), 183198
  Karanth, S.; Delcea, M.
  (See online at https://doi.org/10.1016/j.bbamem.2020.183198)
• NLRP3 Inflammasome Regulates Development of Systemic Inflammatory Response and Compensatory Anti- Inflammatory Response Syndromes in Mice With Acute Pancreatitis. Gastroenterology 2020, 158 (1), 253-269
  Sendler, M.; van den Brandt, C.; Glaubitz, J.; Wilden, A.; Golchert, J.; Weiss, F. U.; Homuth, G.; Chama, L. L. D.; Mishra, N.; Mahajan, U. M.; Bossaller, L; Völker, U.; Bröker, B. M.; Mayerle, J.; Lerch, M. M.
  (See online at https://doi.org/10.1053/j.gastro.2019.09.040)
• Oxidation of Unsaturated Phospholipids: A Monolayer Study. Langmuir 2020, 36(41), 12213-12220
  Geliert, F.; Ahrens, H.; Helm, C. A.
  (See online at https://doi.org/10.1021/acs.langmuir.0c01950)
• Oxidative modification of skin lipids by cold atmospheric plasma (CAP): A standardizable approach using RP-LC/MS2 and DI-ESI/MS2. Chemistry and physics of lipids 2020, 226, 104786
  Striesow, J.; Lackmann, J.-W.; Ni, Z.; Wenske, S.; Weltmann, K.-D.; Fedorova, M.; von Woedtke, T.; Wende, K.
  (See online at https://doi.org/10.1016/j.chemphyslip.2019.104786)
• Pancreatic Remodeling and Fibrosis are Modulated byCD25+/FoxP3+Regulatory T- cells in a Chronic Model of Pancreatitis. Pancreas 2020, 49(10), 1410-1411
  Glaubitz, J.; Wilden, A.; Weiss, F. U.; Mayerle, J.; Lerch, M. M.; Sendler, M.
  
• Pentathiepins: A Novel Class of Glutathione Peroxidase 1 Inhibitors that Induce Oxidative Stress, Loss of Mitochondrial Membrane Potential and Apoptosis in Human Cancer Cells. ChemMedChem 2020, 15(16), 1515-1528
  Behnisch-Cornwell, S.; Bandaru, S. S. M.; Napierkowski, M.; Wolff, L.; Zubair, M.; Urbainsky, C.; Lillig, C.; Schulzke, C.; Bednarski, P. J.
  (See online at https://doi.org/10.1002/cmdc.202000160)
• Proliferation of C6 glioma cells requires the phospholipid remodeling enzyme tafazzin independent of cardiolipin composition. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids 2020, 1865 (3), 158596
  Schild, L.; Döring, M.; Jansing, S.; Peter, D.; Jagirdar, G.; Wolke, C.; Gardemann, A.; Lendeckel, U.
  (See online at https://doi.org/10.1016/j.bbalip.2019.158596)
• Protein Engineering for Enhanced Acyltransferase Activity, Substrate Scope, and Selectivity of the Mycobacterium smegmatis Acyltransferase MsAcT. ACS Catalysis 2020, 10 (14), 7552-7562
  Godehard, S. P.; Badenhorst, C. P. S.; Müller, H.; Bornscheuer, U. T.
  (See online at https://doi.org/10.1021/acscatal.0c01767)
• Receptor-induced Redox Signalling Relay - From Signal to Effector. Free Radical Biology and Medicine 2020, 159, S118-S118
  Ortegón Salas, C.; Knaup, L. K.; Schneider, K.; Scharf, C.; Berndt, C.; Deponte, M.; Lillig, C. H.; Geliert, M.
  (See online at https://doi.org/10.1016/j.freeradbiomed.2020.10.298)
• Role of Electrostatic Interactions for the Prediction of Function and Specificity in Redox Protein-protein Interactions. Free Radical Biology and Medicine 2020, 159, S24-S24
  Hossain, M. F.; Bodnar, Y.; Liebscher, V.; Geliert, M.; Lillig, C. H.
  (See online at https://doi.org/10.1016/j.freeradbiomed.2020.10.073)
• Sequence-Based Prediction of Promiscuous Acyltransferase Activity in Hydrolases. Angewandte Chemie-International Edition 2020, 59 (28), 11607-11612
  Müller, H.; Becker, A. K.; Palm, G. J.; Berndt, L; Badenhorst, C. P. S.; Godehard, S. P.; Reisky, L.; Lammers, M.; Bornscheuer, U. T.
  (See online at https://doi.org/10.1002/anie.202003635)
• Signal-regulated oxidation of proteins via MICAL. Biochemical Society Transactions 2020, 48 (2), 613-620
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