Molecular and functional characterization of cooperating events in acute myeloid leukemia with NPM1 mutation (B03)

• Subject Area: Hematology, Oncology
• Term: from 2012 to 2024
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 217328187

Mutations in the Nucleophosmin gene (NPM1mut) are among the most frequent genetic abnormalities in acute myeloid leukemia (AML). The project aims are: 1) Evaluation of the clinical impact of genetic events cooperating with NPM1 mutations in a unique cohort of 588 patients with NPM1-mutant AML from a randomized clinical trial; 2) analysis of clonal genetic evolution patterns using novel single-cell sequencing technologies; 3) functional analyses of cooperating mutations (e.g., of HNRNPK, PTPN11, MYC, RAD21 genes) by using CRISPR/Cas9 technology; 4) study of the impact of NPM1 mutations on the transcriptome and proteome.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1074: Experimental Models and Clinical Translation in Leukemia
• Applicant Institution: Universität Ulm
• Project Heads: Professor Dr. Lars Bullinger; Professorin Dr. Konstanze Döhner