Molecular and functional characterization of cooperating events in acute myeloid leukemia with NPM1 mutation (B03)

Mutations in the Nucleophosmin gene (NPM1mut) are among the most frequent genetic abnormalities in acute myeloid leukemia (AML). The project aims are: 1) Evaluation of the clinical impact of genetic events cooperating with NPM1 mutations in a unique cohort of 588 patients with NPM1-mutant AML from a randomized clinical trial; 2) analysis of clonal genetic evolution patterns using novel single-cell sequencing technologies; 3) functional analyses of cooperating mutations (e.g., of HNRNPK, PTPN11, MYC, RAD21 genes) by using CRISPR/Cas9 technology; 4) study of the impact of NPM1 mutations on the transcriptome and proteome.

DFG Programme Collaborative Research Centres

Subproject of SFB 1074:  Experimental Models and Clinical Translation in Leukemia

Applicant Institution Universität Ulm

Project Heads Professor Dr. Lars Bullinger; Professorin Dr. Konstanze Döhner