Rasterkraftmikroskop
Final Report Abstract
The instrument (an atomic force microscope combined with an inverted optical microscope) has been used for the following scientific areas/purposes according to the proposal: 1) Mechanical characterization of soft, biological interfaces (membranes, polymers). For the quantitative investigation of morphological dynamics, adhesion and motion of cells and tissue extracts, it is highly important to quantify the mechanical properties of soft interfaces, such as membranes and polymers. Here, we performed either nano-indentation measurements using both commercially available cantilevers as well as particle-modified cantilevers. 2) Topological characterization of soft, biological interfaces. In most of the projects, we functionalize substrates and membranes with various biofunctional molecules, such as extracellular domains of cell adhesion molecules. The combination of atomic force microscopy and fluorescence microscopy is ver ypowerful to confirm the quality of surface functionalization for many different purposes. 3) Mechanical characterization of cells and tissue extracts. Here, we used this instruments to (a) determine the mechanical properties of cells and tissue extracts (e.g. bulk elastic modulus), (b) measure the adhesion force between cells and contact surfaces. As explained in the following sections more in detail, this enables us to perform highly interdisciplinary research, which resulted in publications in top journals.
Publications
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Cell Differentiation of Pluripotent Tissue Sheets Immobilized on Supported Membranes Displaying Cadherin-11. PLoS ONE, 2013. 8(2): e54749
Körner, A., C. Deichmann, F.F. Rossetti, A. Köhler, O.V. Konovalov, D. Wedlich, and M. Tanaka
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Morphology and Adhesion Strength of Myoblast Cells on Photocurable Gelatin under Native and Non-native Micromechanical Environments. The Journal of Physical Chemistry B, 2013. 117(15): 4081-4088
Yoshikawa, H.Y., T. Kawano, T. Matsuda, S. Kidoaki, and M. Tanaka
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High Precision, Electrochemical Detection of Reversible Binding of Recombinant Proteins on Wide Band Gap GaN Electrodes Functionalized with Biomembrane Models. Advanced Functional Materials, 2014. 24(31): 4927-4934
Frenkel, N., J. Wallys, S. Lippert, J. Teubert, S. Kaufmann, A. Das, E. Monroy, M. Eickhoff, and M. Tanaka
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Cytoadhesion of falciparum-infected erythrocytes to chondroitin-4-sulfate is cooperative and shear-enhanced. Blood, 2015, 125, 383–391
Rieger, H., H.Y. Yoshikawa, K. Quadt, M. A. Nielsen, C. Sanchez, A.Salanti, M. Tanaka and M. Lanzer
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Fine Adjustment of Interfacial Potential between pH-Responsive Hydrogels and Cell-Sized Particles. Langmuir, 2015, 31, 8689
Monzel, C., M. Veschgini, J.P. Madsen, A.L. Lewis, S.P. Armes, M. Tanaka
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Generic Roles of Polymer Supports in the Fine Adjustment of Interfacial Interactions between Solid Substrates and Model Cell Membranes. Langmuir, 2015, 31, 4437−4480
Rossetti, F.F., E. Schneck, O. Konovalov, G. Fragneto and M. Tanaka
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Live cell tracking of symmetry break in actin cytoskeleton triggered by abrupt changes in micromechanical environments. Biomaterials Science, 2015, 3(12): 1539-1544
Inoue, S, V. Frank, M. Hörning, S. Kaufmann, H.Y. Yoshikawa, J.P. Madsen, A.L. Lewis, S.P. Armes, M. Tanaka
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Quantifying Adhesion Mechanisms and Dynamics of Human Hematopoietic Stem and Progenitor Cells. Scientific Reports, 2015, 5, 9370
Burk, A.S., C. Monzel, H. Y. Yoshikawa, Wuchter, R. Saffrich, V. Eckstein, M. Tanaka, and A. D. Ho
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Frequent mechanical stress suppresses proliferation of mesenchymal stem cells from human bone marrow without loss of multipotency. Scientific Reports, 2016, 6: 24264
Frank, V., S. Kaufmann, R. Wright, P. Horn, HY. Yoshikawa, P. Wuchter, H.P. Madsen, AL. Lewis, SP. Armes, A.D. Ho, M. Tanaka
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Tracking mechanical and morphological dynamics of regenerating Hydra tissue fragments using a two fingered micro-robotic hand. Applied Physics Letters, 2016, 108(10): 103702
M. Veschgini, F.Gebert, N. Khangai, H. Ito, R. Suzuki, T.W. Holstein, Y. Mae, T. Arai, M. Tanaka
