Project Details
Projekt
Interaction network and regulation of the human ABC transporter MDR3 (B03)
Subject Area
Biophysics
Term
from 2012 to 2020
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 190586431
The human ABC transporter MDR3 catalyses the flop of PC-lipids from the inner to the outer leaflet of the hepatocyte membrane. Together with BSEP, which transports bile acids (BA) and ABCG5/G8 translocating cholesterol, mixed micelles in the canalicular duct. Based on the results of the second funding period, we will continue the studies aimed to unravel the mechanism of PC-lipid transport. Therefore, we will establish an in vitro liposomal-based assay for detailed functional analysis. In parallel, we will analyse the interplay of MDR3 with BSEP or BSEP and ABCG5/G8 in cell culture and the modulation of this transporter network by BA, lipids and/or small molecules.
DFG Programme
Collaborative Research Centres
Applicant Institution
Heinrich-Heine-Universität Düsseldorf
Project Head
Professor Dr. Lutz Schmitt
