Map-based cloning and functional validation of a QTL for grain size in wheat
Final Report Abstract
The genomic region of thousand grain weight QTL Q.Tgw.ipk-7D in wheat was cloned in a BAC contig of 38 sequenced BACs in wheat and 16 sequenced BACs of the syntenic region in barley. Synteny was established to the annotated genome sequences of rice, sorghum and Brachypodium. A total of 16 candidate genes were detected. Further fine mapping and marker development narrowed down the region to the four most likely candidates, one ZINC FINGER PROTEIN 103, a PROTEIN KINASE, an OXIDOREDUCTASE and a MYB factor. These were characterized by expression analysis in developing grains. Functional verification of the target genes by knockout via the RNA-guided nuclease technology has been initiated. The developed near isogenic lines carrying Q.Tgw.ipk-7D showed increased thousand grain weight, grain area and grain length, when tested under field like conditions in a green house. Currently a number of the lines are evaluated for their agronomic performance in the field in collaboration with an industrial partner. Overall, the obtained results can be considered as a major step towards the identification of genes underlying a TGW-QTL in wheat.
Publications
- Grain size QTL region QTgw.ipk-7D in wheat: sequence analysis and synteny to related grass species. GPZ: 10th conference – Plant Stress and Genomics, Halle/Saale, 18.-20.09. 2012
Jaenecke, C., N. Weichert, H. Ŝimková, J. Doležel, D. Wolff, U. Scholz, A. Himmelbach, N. Stein, M.S. Röder
- Grain size QTL region QTgw.ipk-7D in wheat: sequence analysis and synteny to related grass species. Workshop Molekulare Präzisionszüchtung, Gatersleben, 12.-13.09.2013
Jaenecke, C., C. Zanke, N. Weichert, H. Ŝimková, J. Doležel, D. Wolff, U. Scholz, A. Himmelbach, N. Stein, M.S. Röder
- Grain size QTL region QTgw.ipk-7D in wheat: sequence analysis and synteny to related grass species. EUCARPIA conference, Wernigerode, 29.06.-04.07. 2014
Jaenecke, C., N. Weichert, H. Ŝimková, H. Staňková, J. Doležel, D. Wolff, U. Scholz, A. Himmelbach, N. Stein, M.S. Röder
