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RNP complexes regulating the accessibility of higher order structures of chromatin (B02)

Fachliche Zuordnung Biochemistry
Förderung Förderung von 2011 bis 2019
Projektkennung Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 185476941
 
Chromatin is stably associated with RNA and RNP complexes can open higher order structures of chromatin. We study the functional role of the RNA and the mechanisms of chromatin opening. We perform ChIP-Seq and RNA IPs of DF31 and HMGN5 to identify the chromatin targeting signals and test them in interaction assays. We will functionally address the chromatin opening mechanism with an in vivo GFP-HMGN5 targeting system and mutants of the protein. To study RNA dependent chromatin opening in a genome wide scale, we established the differential MNase treatment of cells combined with high-throughput sequencing and advanced bioinformatic analysis. We will continue to work on the development of the bioinformatical and statistical analysis of the large scale data sets and combine the assay with the functional testing of our model protein.
DFG-Verfahren Collaborative Research Centres
Applicant Institution Universität Regensburg
 
 

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