Substrate recognition by the HRD-ubiquitin ligase (B05)

• Subject Area: Biochemistry
• Term: from 2011 to 2018
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 25065445

One of the best-studied ubiquitin ligases involved in protein quality control is the HMG-CoA reductase degradation-(HRD-) ligase. It is embedded in the endoplasmic reticulum (ER) membrane and constitutes the central component of a process termed ER-Associated Protein Degradation (ERAD). We want to investigate the interaction of the main components of this Module with misfolded client proteins and with the folding machinery of the ER. Besides biochemical in vitro studies and yeast genetics, this includes cryoEM and a CLMS approach.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 740: From Molecules to Modules: Organisation and Dynamics of Functional Units in Cells
• Applicant Institution: shared FU Berlin and HU Berlin through:; Charité - Universitätsmedizin Berlin
• Co-Applicant Institution: Max-Delbrück-Centrum für Molekulare Medizin (MDC)
• Project Heads: Dr. Ernst Jarosch, until 12/2014; Professor Dr. Thomas Sommer