High-resolution structure determination of dynamic macromolecular complexes by cryo-EM (A05)

• Subject Area: Structural Biology
• Term: from 2010 to 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 105286809

The planned work program includes the high-resolution 3D structure determination of several macromolecular complexes, which are current projects in the Collaborative Research Centres. The focus for the next funding period will be on structure determination of further intermediate states of the dynamic spliceosome. The quantitative analysis of the dynamics of the spliceosome is of particular interest, for which we have planned methodological work to improve computer-aided image processing procedures. Another project is the structural analysis of the human 48S Translation-Intitiations complex.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 860: Integrative Structural Biology of Dynamic Macromolecular Assemblies
• Applicant Institution: Georg-August-Universität Göttingen
• Project Head: Professor Dr. Holger Stark