Biochemical dissection, functional characterization and regulatory cues of the assembly machinery for U snRNPs

• Applicants: Professor Dr. Utz Fischer; Professor Dr. Oliver Gruß
• Subject Area: Cell Biology
• Term: from 2009 to 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 119111219

• Final Report Year: 2021

Final Report Abstract

No abstract available

Publications

• ProteoPlex: stability optimization of macromolecular complexes by sparse-matrix screening of chemical space (2015). Nature Methods 12 (9), 859-865
  A Chari, D Haselbach, JM Kirves, J Ohmer, E Paknia, N Fischer, O Ganichkin, V Möller, JJ Frye, G Petzold, M Jarvis, M Tietzel, C Grimm, JM Peters, BA Schulman, K Tittmann, J Markl, U Fischer and H Stark
  (See online at https://doi.org/10.1038/nmeth.3493)
• The ribosome cooperates with the assembly chaperone pICln to initiate formation of snRNPs (2016). Cell Reports 16 (12), 3103-3112
  E Paknia, A Chari, H Stark and U Fischer
  (See online at https://doi.org/10.1016/j.celrep.2016.08.047)
• Deciphering the mRNP code: RNA-bound determinants of post-transcriptional gene regulation (2017). TIBS 42 (5), 369-382
  NH Gehring, E Wahle, U Fischer
  (See online at https://doi.org/10.1016/j.tibs.2017.02.004)
• Impaired spliceosomal UsnRNP assembly leads to Sm mRNA down-regulation and Sm protein degradation (2017). Journal of Cell Biology 216 (8), 2391-2407
  AB Prusty, R Meduri, BK Prusty, J Vanselow, A Schlosser and U Fischer
  (See online at https://doi.org/10.1083/jcb.201611108)
• UsnRNP biogenesis: mechanisms and regulation (2017). Chromosoma 126 (5), 577-593
  OJ Gruss, R Meduri, M Schilling and U Fischer
  (See online at https://doi.org/10.1007/s00412-017-0637-6)
• Developmental epileptic encephalopathy with hypomyelination and brain atrophy associated with PTPN23 variants affecting the assembly of UsnRNPs (2018). Eur J Hum Genet 26 (10), 1502-1511
  L Smigiel, G Landsberg, M Schilling, M Rydzanicz, K Pollak, A Walczak, A Stodolak, P Stawinski, H Mierzewska, MM Sasiadek, OJ Gruss and R Ploski
  (See online at https://doi.org/10.1038/s41431-018-0179-2)
• A missense mutation in SNRPE linked to non-syndromal microcephaly interferes with U snRNP assembly and pre-mRNA splicing (2019). PLoS genetics 15 (10), e1008460
  T Chen, B Zhang, T Ziegenhals, AB Prusty, S Fröhler, C Grimm, Y Hu, B Schaefke, L Fang, M Zhang, N Kraemer, A Kaindl, U Fischer and W Chen
  (See online at https://doi.org/10.1371/journal.pgen.1008460)
• TOR signaling regulates liquid phase separation of the SMN complex governing snRNP biogenesis (2021) Cell Reports. 2021 Jun 22;35(12):109277
  M Schilling, A Prusty, B Boysen, F Oppermann, Y Riedel, A Husedzinovic, H Rasouli, A König, J Reymann, H Erfle, H Daub, U Fischer and OJ Gruss
  (See online at https://doi.org/10.1016/j.celrep.2021.109277)